Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Inhibition rate of cancer cell under taking TGL in Kunming mice. (a) The tumor of mice bearing S180 and H22 cells. (b) Tumor weight. (c) Tumor inhibition rate. n = 12. The different lowercase and uppercase letters means significant difference between S180 and H22 cells respectively, p < 0.05.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques: Inhibition

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Effect of TGL on immune organ and organ index in mice. (a) Spleen and thymus of mice bearing S180 and H22 cells. (b) Spleen index. (c) Thymus index. n = 12. The different lowercase and uppercase letters means significant difference between S180 and H22 cells respectively, p < 0.05.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques:

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Effect of TGL on the antioxidant components in the serum of mice bearing S180 and H22 cells. (a) SOD (mmol/L). (b) CAT (mmol/L). (c) MDA (mmol/L). n = 12. The different lowercase and uppercase letters means significant difference between S180 and H22 cells respectively, p < 0.05.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques:

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Expression of Bcl-2 and Bax protein in S180 and H22 tumor and gut by TGL treatment. (a) Expression of Bcl-2 and Bax protein in S180 and H22 tumor. (b) Expression of Bcl-2 and Bax protein in gut. n = 12. The different lowercase and uppercase letters means significant difference between Bcl-2 and Bax protein expression respectively, p < 0.05. Original images of western blot were shown in .

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques: Expressing, Western Blot

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Sequencing data and the alpha diversity in each group of mice (n = 12).

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques: Sequencing

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Distribution of gut microbiota at different level. (a) Analysis of the composition of bacteria at the genus level of S180 tumor-bearing mice. (b) Analysis of the composition of bacteria at the genus level of H22 tumor-bearing mice. (c) 3D-PCoA of the gut microbiota of S180 tumor-bearing mice. (d) 3D-PCoA of the gut microbiota of H22 tumor-bearing mice. n = 12.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques: Bacteria

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Line chart of species classification box. (a) Gut microbiota of S180 tumor-bearing mice. (b) Gut microbiota of H22 tumor-bearing mice.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques:

Journal: BMC Cancer

Article Title: The spectrum of resistance in SR/CR mice: the critical role of chemoattraction in the cancer/leukocyte interaction

doi: 10.1186/1471-2407-10-179

Figure Lengend Snippet: SR/CR mice resist a broad range of lethal cancer cell lines at different doses . The percentage of SR/CR mice that were able to survive challenge with J774, MethA, EL-4, S180, B16, or LL/2 was determined empirically. Mice were monitored for at least 60 days after challenge. SR/CR C57BL/6 mice were challenged with 10e3 or 5 × 10e4 B16 or LL/2. SR/CR C57BL/6 mice were also challenged with 0.8 × 10e7, 1.6 × 10e8, 2.2 × 10e8, or 3.2 × 10e8 S180 or 2.5 × 10e7, 5 × 10e7, 10e8, or 2 × 10e8 EL-4. SR/CR BALB/c mice were challenged with 10e5, 2 × 10e5, 4 × 10e5, or 10e6 MethA or 5 × 10e4, 10e6, or 10e7 J774. WT mice uniformly died at all doses with all cell lines tested. At least 5 mice were tested for any given dose of tumor cells injected.

Article Snippet: S180, EL-4, LL/2, B16, and J774 cell lines were purchased from American Type Culture Collection and propagated in culture according to the manufacturer's protocol.

Techniques: Injection

Journal: BMC Cancer

Article Title: The spectrum of resistance in SR/CR mice: the critical role of chemoattraction in the cancer/leukocyte interaction

doi: 10.1186/1471-2407-10-179

Figure Lengend Snippet: Naïve SR/CR pups resist the initial challenge with EL-4 and J774 . Pups from an SR/CR × WT cross were challenged with 10e6 EL-4 prior to being challenged with any other cancer cell line and 22 out of 35 pups survived. Pups from an SR/CR × WT cross that were challenged with 5 × 10e5 and a second challenge with 5 × 10e6 S180 have a historical survival rate of ~30% [ , ]. When nine pups from an SR/CR × WT cross were challenged with 5 × 10e4 J774 prior to being challenged with any other cell line, one mouse survived. All WT mice challenged with EL-4, S180, and J774 at the indicated doses uniformly died. Mice were monitored for at least 60 days after challenge.

Article Snippet: S180, EL-4, LL/2, B16, and J774 cell lines were purchased from American Type Culture Collection and propagated in culture according to the manufacturer's protocol.

Techniques:

Journal: BMC Cancer

Article Title: The spectrum of resistance in SR/CR mice: the critical role of chemoattraction in the cancer/leukocyte interaction

doi: 10.1186/1471-2407-10-179

Figure Lengend Snippet: S180 or cell free ascites fluid (CFAF) enhances SR/CR resistance . SR/CR or WT mice were challenged with 2 × 10e5 LL/2 (upper panel) or 10e6 MethA (lower panel) i.p. either alone or with 10e6 S180, 10e6 irradiated S180, or weekly injections of CFAF and survival curves were generated. The number of mice in each group is indicated in the figure.

Article Snippet: S180, EL-4, LL/2, B16, and J774 cell lines were purchased from American Type Culture Collection and propagated in culture according to the manufacturer's protocol.

Techniques: Irradiation, Generated

Journal: BMC Cancer

Article Title: The spectrum of resistance in SR/CR mice: the critical role of chemoattraction in the cancer/leukocyte interaction

doi: 10.1186/1471-2407-10-179

Figure Lengend Snippet: S180 only enhances SR/CR resistance locally . SR/CR mice were challenged with 2 × 10e5 LL/2 i.p . either alone or with 10e6 S180, either i.p . or s.c ., and the survival of mice was monitored. The number of mice in each group is indicated in the figure.

Article Snippet: S180, EL-4, LL/2, B16, and J774 cell lines were purchased from American Type Culture Collection and propagated in culture according to the manufacturer's protocol.

Techniques:

Journal: BMC Cancer

Article Title: The spectrum of resistance in SR/CR mice: the critical role of chemoattraction in the cancer/leukocyte interaction

doi: 10.1186/1471-2407-10-179

Figure Lengend Snippet: SR/CR leukocyte migration and cancer destruction . A proposed model for SR/CR mediated cancer cell killing. S180 secretes chemotactic factors that attract SR/CR leukocytes (SR) to the site of the cancer, allowing tight contact between the leukocytes and cancer cells to be made which facilitates tumor destruction. LL/2 and MethA produce little or no chemoattractant and are, therefore, not efficiently killed by SR/CR leukocytes. Co-injection of MethA or LL/2 with S180 results in attraction of SR/CR leukocytes to the site of the cancer and efficient cell killing of these cancer cell lines. This suggests that some cancer cells may escape from SR/CR resistance because they do not induce SR/CR leukocyte infiltration and demonstrates the importance of leukocyte infiltration in the SR/CR resistance mechanism.

Article Snippet: S180, EL-4, LL/2, B16, and J774 cell lines were purchased from American Type Culture Collection and propagated in culture according to the manufacturer's protocol.

Techniques: Migration, Injection

Journal: Cancer cell

Article Title: Th9 Cells Represent a Unique Subset of CD4 + T Cells Endowed with the Ability to Eradicate Advanced Tumors

doi: 10.1016/j.ccell.2018.05.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-mouse p-IKKα/β (S180/181) , Santa Cruz Biotechnology , sc-23470-R.

Techniques: Blocking Assay, Staining, Extraction, Enzyme-linked Immunosorbent Assay, Luminescence Assay, Recombinant, shRNA, Negative Control, Binding Assay, Modification, Software, Gene Expression